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Updated: Mar 16, 2026

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

[Study on Salmonella serotyping by use of Microsphere-based Liquid Array method].

B X Ke¹, D M He¹, H L Tan¹

¹Institute of Pathogenic Microorganism, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.

Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi

|August 20, 2016

Summary

This summary is machine-generated.

The Microsphere-based Liquid Array (SSA) method effectively serotyped over 96% of Salmonella strains in Guangdong. This molecular technique proved faster and more accurate than traditional methods for Salmonella serotyping.

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A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

Area of Science:

Microbiology
Molecular Biology
Public Health

Background:

Salmonella serotyping is crucial for tracking foodborne illness outbreaks.
Traditional serotyping methods can be time-consuming and may not identify all strains.
Molecular methods offer potential for improved accuracy and speed.

Purpose of the Study:

To evaluate the effectiveness of the Microsphere-based Liquid Array (SSA) method for Salmonella serotyping.
To compare the SSA method with traditional serotyping techniques in Guangdong province.
To assess the impact of serotyping on Salmonella isolates from diarrhea patients.

Main Methods:

Salmonella strains isolated from human diarrhea patients in Guangdong were analyzed.
The Microsphere-based Liquid Array (SSA) platform and SSA kit were utilized for serotyping.
Molecular serotyping was compared against traditional methods.

Main Results:

Over 4,942 Salmonella strains, representing 189 serotypes, were identified between 2010-2014.
The SSA kit achieved high concordance rates for O antigen (100%) and H antigen (98.32%).
Molecular serotyping successfully identified 11 strains that were untypeable by traditional methods.

Conclusions:

The SSA kit enables efficient serotyping of over 96% of Salmonella strains in Guangdong.
Molecular serotyping via SSA demonstrates a concordance rate exceeding 98% compared to traditional methods.
The Microsphere-based Liquid Array technique provides a faster and more accurate approach to Salmonella serotyping.