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Cell surface protein antigen from Wolinella recta ATCC 33238T
S Kokeguchi1, K Kato, H Kurihara
1Department of Oral Microbiology, Okayama University Dental School, Japan.
Journal of Clinical Microbiology
|June 1, 1989
Summary
A novel high-molecular-weight protein from Wolinella recta is strongly recognized by sera from periodontitis patients, suggesting its potential role in periodontitis pathogenesis.
Area of Science:
- Microbiology
- Immunology
- Periodontal Disease Research
Background:
- Wolinella recta is a bacterium associated with periodontal disease.
- Identifying specific bacterial antigens involved in periodontitis is crucial for understanding disease mechanisms.
Purpose of the Study:
- To isolate and characterize a specific protein antigen from Wolinella recta.
- To investigate the reactivity of this protein with sera from periodontitis patients and healthy individuals.
Main Methods:
- Acid extraction and purification of a high-molecular-weight protein from Wolinella recta.
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein analysis.
- Immunoblotting and enzyme-linked immunosorbent assay (ELISA) for antibody detection.
Main Results:
- A 150,000 molecular weight protein was isolated from Wolinella recta.
- This protein was specific to W. recta and not found in other Wolinella species.
- Sera from periodontitis patients showed significantly higher reactivity to the protein compared to healthy donors.
Conclusions:
- The isolated protein is a potential Wolinella recta-specific antigen.
- This antigen may play a role in the immune response associated with periodontitis.