Related Experiment Video
Updated: Jul 16, 2026

Identification of Fatty Acids in Bacillus cereus
Published on: December 5, 2016
Quantification of Bacterial Fatty Acids by Extraction and Methylation
Mark Politz1, Rebecca Lennen1, Brian Pfleger1
1Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA.
Abstract:
This protocol describes two similar methods for the extraction and methylation of fatty acids from bacterial cultures. The acid derivatization protocol (Lennen et al., 2013; Bligh and Dyer, 1959) results in the extraction and methylation of all fatty acids, both free and bound, from a bacterial culture, while the base derivatization protocol (Lennen and Pfleger, 2013) captures only bound (phospholipid, acyl-thioester) species. After extraction into hexane, the lipids may be analyzed by gas chromatography.
More Related Videos
11:14Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
Published on: April 17, 2018
07:34Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide
Published on: May 12, 2023