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Biodegradation and Osteosarcoma Cell Cultivation on Poly(aspartic acid) Based Hydrogels
Dávid Juriga1, Krisztina Nagy2, Angéla Jedlovszky-Hajdú1
1Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University , Nagyvárad tér 4., H-1089 Budapest, Hungary.
ACS Applied Materials & Interfaces
|August 20, 2016
Summary
Biodegradable poly(aspartic acid) hydrogels support osteoblast-like cell growth. These novel scaffolds show promise for preclinical and clinical cell cultivation applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Polymer Chemistry
Background:
- Developing biodegradable and biocompatible scaffolds is crucial for regenerative medicine.
- Poly(aspartic acid) (PASP) hydrogels offer potential due to their tunable properties.
Purpose of the Study:
- To evaluate the biodegradability of poly(aspartic acid)-based hydrogels.
- To assess the suitability of these hydrogels as scaffolds for MG-63 osteoblast-like cells.
Main Methods:
- Fabrication of PASP hydrogels using natural amines as cross-linkers.
- Modification of hydrogels with disulfide bridges, thiol groups, and RGD sequences.
- Assessment of biodegradability using enzymes and cell culture medium; cell viability and morphology studies with MG-63 cells.
Main Results:
- PASP hydrogels with disulfide cross-links were degradable by collagenase I.
- MG-63 cells exhibited healthy morphology on double cross-linked, RGD-modified hydrogels.
- Thiolated PASP hydrogels promoted cell adhesion, survival, proliferation, and migration, with RGD influencing cell clustering.
Conclusions:
- Biodegradable and biocompatible thiolated poly(aspartic acid) hydrogels are promising for osteoblast-like cell cultivation.
- These materials provide optimal conditions for cell adhesion, survival, proliferation, and migration.
- The RGD motif impacts cell behavior, suggesting potential for controlled tissue regeneration.
