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Related Concept Videos

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

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Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
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Nuclear Localization Signals and Import01:46

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Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
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Subcellular Fractionation01:32

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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
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Regulation of Nuclear Protein Sorting01:45

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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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Regulated mRNA Transport02:22

Regulated mRNA Transport

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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
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RNALocate: a resource for RNA subcellular localizations.

Ting Zhang1, Puwen Tan1, Liqiang Wang1

  • 1College of Bioinformatics Science and Technology, Harbin Medical University, Harbin 150081, China.

Nucleic Acids Research
|August 21, 2016
PubMed
Summary
This summary is machine-generated.

RNALocate is a database detailing RNA subcellular localization, crucial for understanding RNA functions. It offers over 37,700 curated entries across 65 species to aid RNA research.

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Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • RNA subcellular localization is vital for understanding biological functions.
  • Accurate localization data is essential for RNA research and function prediction.
  • Existing resources may lack comprehensive, manually curated localization data.

Purpose of the Study:

  • To establish RNALocate, a comprehensive, web-accessible database for RNA subcellular localization.
  • To provide a high-quality resource with manually curated entries and experimental evidence.
  • To facilitate future research on RNA function, structure, and localization prediction.

Main Methods:

  • Manual curation of RNA subcellular localization data from scientific literature.
  • Integration of RNA homology, sequence, and interaction data.
  • Development of user-friendly access tools including search, browse, BLAST, and visualization.

Main Results:

  • RNALocate version 1.0 contains over 37,700 manually curated entries.
  • Data covers more than 21,800 RNAs across 42 subcellular localizations and 65 species.
  • Includes major species like Homo sapiens, Mus musculus, and Saccharomyces cerevisiae.

Conclusions:

  • RNALocate provides a valuable, experimentally supported resource for RNA subcellular localization.
  • The database aids in understanding the entirety of RNA localization patterns.
  • RNALocate can support the development of novel RNA localization prediction methods.