Related Experiment Video
Updated: Mar 16, 2026
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
Hepatitis C virus G1b infection decreases the number of small low-density lipoprotein particles
Chika Kinoshita1, Tomohisa Nagano1, Nobuyoshi Seki1
1Chika Kinoshita, Tomohisa Nagano, Nobuyoshi Seki, Yoichi Tomita, Tomonori Sugita, Yuta Aida, Munenori Itagaki, Kenichi Satoh, Satoshi Sutoh, Hiroshi Abe, Yoshio Aizawa, Department of Gastroenterology and Hepatology Internal Medicine of Jikei University Katsushika Medical Center, Tokyo 125-8506, Japan.
Insights
Hepatitis C virus (HCV) G1b infection significantly lowers the particle numbers of medium, small, and very small low-density lipoprotein (LDL) particles. This finding highlights a specific impact of active HCV G1b infection on lipoprotein profiles.
Area of Science:
- Virology
- Lipid Metabolism
- Biochemistry
Background:
- Hepatitis C virus (HCV) infection is a global health concern.
- Understanding the impact of HCV on host lipid metabolism is crucial for managing associated complications.
- Previous studies have suggested alterations in lipoprotein profiles in HCV patients, but specific impacts of genotype 1b require further investigation.
Purpose of the Study:
- To investigate the influence of hepatitis C virus (HCV) G1b infection on the particle number of various lipoproteins.
- To determine if active HCV G1b infection is associated with specific changes in lipoprotein sub-fractions compared to sustained virologic response (SVR).
Main Methods:
- Serum lipoprotein particle numbers were quantified in 173 Japanese subjects (82 active HCV G1b, 91 SVR) using high-performance liquid chromatography and a novel algorithm.
- Cholesterol and triglyceride concentrations in lipoprotein fractions were measured using LipoSEARCH.
- Multiple linear regression analysis was employed to assess the relationship between HCV G1b infection and lipoprotein particle numbers.
Main Results:
- Active HCV G1b infection was associated with significantly lower numbers of total low-density lipoprotein (LDL) particles compared to the SVR group.
- Specifically, the particle numbers of medium, small, and very small LDL sub-fractions were significantly reduced in individuals with active HCV G1b infection.
- No significant differences were observed in very low-density lipoprotein (VLDL) or high-density lipoprotein (HDL) particle numbers, nor in large LDL particles, between the groups.
Conclusions:
- Hepatitis C virus (HCV) G1b infection is associated with a significant decrease in the particle numbers of medium, small, and very small LDL.
- These findings suggest a specific impact of active HCV G1b infection on LDL metabolism, independent of HDL and VLDL levels.
Aim:
To investigate how hepatitis C virus (HCV) G1b infection influences the particle number of lipoproteins.
Methods:
The numbers of lipoprotein particles in fasting sera from 173 Japanese subjects, 82 with active HCV G1b infection (active HCV group) and 91 with cleared HCV infection (SVR group), were examined. Serum lipoprotein was fractionated by high-performance liquid chromatography into twenty fractions. The cholesterol and triglyceride concentrations in each fraction were measured using LipoSEARCH. The number of lipoprotein particles in each fraction was calculated using a newly developed algorithm, and the relationship between chronic HCV G1b infection and the lipoprotein particle number was determined by multiple linear regression analysis.
Results:
The median number of low-density lipoprotein (LDL) particles was significantly lower in the active HCV group [1182 nmol/L, interquartile range (IQR): 444 nmol/L] than in the SVR group (1363 nmol/L, IQR: 472 nmol/L, P < 0.001), as was that of high-density lipoprotein (HDL) particles (14168 nmol/L vs 15054 nmol/L, IQR: 4114 nmol/L vs 3385 nmol/L, P = 0.042). The number of very low-density lipoprotein (VLDL) particles was similar between the two groups. Among the four LDL sub-fractions, the number of large LDL particles was similar between the two groups. However, the numbers of medium (median: 533.0 nmol/L, IQR: 214.7 nmol/L vs median: 633.5 nmol/L, IQR: 229.6 nmol/L, P < 0.001), small (median: 190.9 nmol/L, IQR: 152.4 nmol/L vs median: 263.2 nmol/L, IQR: 159.9 nmol/L; P < 0.001), and very small LDL particles (median: 103.5 nmol/L, IQR: 66.8 nmol/L vs median: 139.3 nmol/L, IQR: 67.3 nmol/L, P < 0.001) were significantly lower in the active HCV group than in the SVR group, respectively. Multiple linear regression analysis indicated an association between HCV G1b infection and the decreased numbers of medium, small, and very small LDL particles. However, active HCV infection did not affect the number of large LDL particles or any sub-fractions of VLDL and HDL particles.
Conclusion:
HCV G1b infection decreases the numbers of medium, small, and very small LDL particles.
Related Concept Videos
01:20Lipid-Lowering Drugs: Statins and Miscellaneous Agents
01:29Viruses with RNA Genomes
01:26Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow