Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Survival Benefit and Prognostic Determinants of Conversion Surgery in Patients With Stage IV Gastric Cancer: A Single-center Study.

Anticancer research·2026
Same author

Efficacy and Safety of First-Line Ramucirumab Plus Erlotinib for <i>EGFR</i> L858R-Mutated NSCLC in Real-World Practice: A Retrospective Multicenter REAL-SPEED Analysis.

JTO clinical and research reports·2026
Same author

Correction to: Decompressive craniectomy following malignant cerebral infarction is an independent risk factor for ventriculomegaly.

Neurosurgical review·2026
Same author

Quantitative dissection of sexual dimorphism in mice through Y-linked gene knockouts and multivariate phenotyping.

Scientific reports·2026
Same author

Multifocal Intracranial Cryptococcoma with Treatment Resistance, Recurrence, and Mortality: A Case Report and Literature Review.

NMC case report journal·2025
Same author

Evaluation of severe and chronic factors for extended stays in Japanese medical treatment and supervision act wards.

Frontiers in psychiatry·2025

Related Experiment Video

Updated: Mar 16, 2026

Author Spotlight: EasyFlow - An Economical and Adaptable Perfusion Bioreactor for Large Blood Vessel Culture
06:44

Author Spotlight: EasyFlow - An Economical and Adaptable Perfusion Bioreactor for Large Blood Vessel Culture

Published on: July 28, 2023

4.7K

Self-driven perfusion culture system using a paper-based double-layered scaffold.

Ai Ozaki, Yoshinori Arisaka, Naoya Takeda

    Biofabrication
    |August 24, 2016
    PubMed
    Summary

    A novel paper-based scaffold enables self-driven perfusion culture for engineered tissues. This system uses capillary action and siphoning for stable flow, promoting cell growth and orientation for future 3D tissue fabrication.

    More Related Videos

    Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography
    11:12

    Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography

    Published on: October 3, 2018

    6.1K
    Elastomeric PGS Scaffolds in Arterial Tissue Engineering
    08:35

    Elastomeric PGS Scaffolds in Arterial Tissue Engineering

    Published on: April 8, 2011

    16.2K

    Related Experiment Videos

    Last Updated: Mar 16, 2026

    Author Spotlight: EasyFlow - An Economical and Adaptable Perfusion Bioreactor for Large Blood Vessel Culture
    06:44

    Author Spotlight: EasyFlow - An Economical and Adaptable Perfusion Bioreactor for Large Blood Vessel Culture

    Published on: July 28, 2023

    4.7K
    Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography
    11:12

    Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography

    Published on: October 3, 2018

    6.1K
    Elastomeric PGS Scaffolds in Arterial Tissue Engineering
    08:35

    Elastomeric PGS Scaffolds in Arterial Tissue Engineering

    Published on: April 8, 2011

    16.2K

    Area of Science:

    • Biomaterials Engineering
    • Tissue Engineering
    • Microfluidics

    Background:

    • Fluid shear stress influences cell development in vivo.
    • Perfusion culture is crucial for in vitro tissue engineering, especially for vascularized tissues.
    • Conventional microfluidic perfusion systems have limitations in culture area and complexity.

    Purpose of the Study:

    • To develop a novel, self-driven perfusion culture system for engineered tissues.
    • To overcome limitations of conventional microfluidic perfusion methods.
    • To investigate the application of shear stress in engineered tissue development.

    Main Methods:

    • Developed a paper-based, double-layered scaffold with electrospun gelatin microfibers.
    • Utilized capillary action and siphon phenomenon for self-driven medium flow between two chambers.
    • Cultivated endothelial cells on the scaffold to assess flow effects.

    Main Results:

    • Achieved stable, constant, and quantitatively controllable medium flow rates.
    • Demonstrated scalability of the culture area to the cm(2) scale.
    • Observed endothelial cell orientation along the medium flow, indicating effective shear stress application.

    Conclusions:

    • The paper-based scaffold system offers a simple yet effective method for self-driven perfusion culture.
    • This system is suitable for fabricating large-scale, three-dimensional engineered tissues.
    • The findings highlight the potential for shear stress-mediated tissue development in engineered constructs.