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Related Experiment Video

Updated: Mar 16, 2026

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
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High-Throughput Sequencing Reveals Single Nucleotide Variants in Longer-Kernel Bread Wheat.

Feng Chen1, Zibo Zhu1, Xiaobian Zhou1

  • 1National Key Laboratory of Wheat and Maize Crop, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural University Zhengzhou, China.

Frontiers in Plant Science
|August 24, 2016
PubMed
Summary

This study compared single nucleotide variants (SNVs) in bread wheat Yunong 201 and its derivative Yunong 3114. Identified SNVs in genes related to ATP binding and protein phosphorylation may explain Yunong 3114's longer kernel length.

Keywords:
ethyl methanesulfonate (EMS)mutationnext-generation sequencingsingle nucleotide variations (SNVs)transcriptomewheat

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Area of Science:

  • Plant genomics
  • Wheat functional genomics
  • Transcriptome analysis

Background:

  • Bread wheat (Triticum aestivum) is a vital global crop.
  • Understanding genetic variations is crucial for crop improvement.
  • Ethyl methanesulfonate (EMS) is a common mutagen used in plant breeding.

Purpose of the Study:

  • To identify and compare single nucleotide variants (SNVs) between bread wheat Yunong 201 and its EMS-induced mutant Yunong 3114.
  • To investigate the functional implications of identified SNVs in wheat transcriptome.
  • To explore the genetic basis for phenotypic differences, such as kernel length.

Main Methods:

  • Next-generation sequencing (NGS) to obtain transcriptomes of Yunong 201 and Yunong 3114.
  • Bioinformatic analysis to identify and compare non-synonymous SNVs.
  • Gene ontology (GO) enrichment analysis to determine biological processes associated with SNVs.
  • Heat map analysis to assess differential gene expression patterns.

Main Results:

  • A total of 5907 and 6287 non-synonymous SNVs were identified in Yunong 201 and Yunong 3114, respectively.
  • 4021 genes contained SNVs, significantly enriched in functions like ATP binding, protein phosphorylation, and cellular protein metabolic processes.
  • Heat map analysis revealed significant differential expression of mutant genes across developmental stages.
  • SNVs in these genes are hypothesized to contribute to the longer kernel length observed in Yunong 3114.

Conclusions:

  • The study provides a comprehensive analysis of SNVs in two bread wheat strains.
  • Identified genes with non-synonymous SNVs are functionally linked to key biological processes.
  • The findings offer valuable insights into wheat functional genomics and the genetic basis of phenotypic variation.