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Published on: March 9, 2021
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HSP90.2 modulates 2Q2-mediated wheat resistance against powdery mildew
Yan Yan1,2, Yue-Ting Guo2,3, Chao-Yan Chang2,3
1National Key Laboratory of Wheat and Maize Crop Science/CIMMYT-China Wheat and Maize Improvement Joint Center/College of Agronomy, Henan Agricultural University, Zhengzhou, China.
Plant, Cell & Environment
|March 9, 2023
Summary
Wheat Heat Shock Protein 90.2 (HSP90.2) is crucial for plant defense against powdery mildew. This study identified over 1500 HSP90.2 clients, revealing its role in pathogen resistance and protein regulation.
Area of Science:
- Plant Molecular Biology
- Plant Pathology
- Biochemistry
Background:
- Wheat (Triticum aestivum L.) production is threatened by pathogens, impacting global food security.
- Heat Shock Protein 90.2 (HSP90.2) is a pathogen-inducible molecular chaperone involved in protein folding.
- Understanding HSP90.2's role is vital for developing strategies to enhance wheat's resistance to diseases.
Purpose of the Study:
- To investigate the function of wheat HSP90.2 in pathogen resistance.
- To identify and characterize clients regulated by HSP90.2 at the posttranslational level.
- To explore the potential of the HSP90.2 interactome in combating fungal pathogens like powdery mildew.
Main Methods:
- Generation of tetraploid wheat hsp90.2 knockout and overexpression lines.
- Isolation and characterization of over 1500 HSP90.2 client proteins.
- Utilizing a 2Q2 nucleotide-binding leucine repeat-rich protein as a model client.
- Creating transgenic lines co-suppressing 2Q2 to assess its role in resistance.
Main Results:
- HSP90.2 is essential for wheat resistance against powdery mildew; knockout mutants were susceptible, while overexpression lines were resistant.
- Over 1500 HSP90.2 clients were identified, indicating its broad role in protein regulation.
- Suppression of 2Q2 increased susceptibility to powdery mildew, identifying 2Q2 as a novel powdery mildew-resistance gene.
- HSP90.2 is critical for the accumulation of chloroplast-localized 2Q2 protein in thylakoids.
Conclusions:
- Wheat HSP90.2 plays a significant role in plant defense mechanisms against fungal pathogens.
- The study provides a comprehensive list of HSP90.2 clients, offering insights into protein folding regulation during pathogenesis.
- This work presents a novel approach to identify pathogenesis-related proteins through HSP90.2 interactome analysis.
