Related Experiment Video
Updated: Mar 16, 2026

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
Published on: September 10, 2013
Photophysical Properties of Fluorescent Core Dendrimers Controlled by Size
Valentina Paolucci1, Søren L Mejlsøe1, Mario Ficker1
1Department of Chemistry, University of Copenhagen , Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
Abstract:
A series of different generation PAMAM dendrimers with sulforhodamine B covalently attached to the dendrimer core was investigated regarding their optical properties. Steady-state and time-resolved spectroscopic techniques were used to determine the size influence of the dendrimers on the photophysical behavior of the luminescent core. New blue emissive species were formed as the generation increased from zero to four. The growth of the dendritic branches resulted in a rise of fluorescence quantum yield and fluorescence lifetime values. Rotational correlation times were used to determine the hydrodynamic diameters of the fluorescent-core dendrimers, and good accordance was found with the values previously reported for unlabeled PAMAM dendrimers, which makes them potentially suitable diagnostic tools for biomedical tracing.
Related Concept Videos
Variables Affecting Phosphorescence and Fluorescence
Super-resolution Fluorescence Microscopy
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Photoluminescence: Applications
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescence and Phosphorescence: Instrumentation

