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Published on: September 27, 2022
Cryopreservation of whole bovine ovaries: comparisons of different thawing protocols
Hai-Hua Gao1, Zong-Peng Li2, Hong-Ping Wang3
1Center for Reproductive Medicine, Hospital for Maternity and Child Care of Linyi City, China.
Objective:
The aim of this study was to perform a comparative investigation of several different thawing protocols and to determine an appropriate protocol for thawing whole bovine frozen ovaries.
Study Design:
Bovine ovaries were slowly frozen and then thawed by applying different protocols. Ultrastructural change, follicle viability, and the hormone levels of culture supernatant were measured.
Results:
The percentage of morphologically normal primordial follicles and the hormone levels of culture supernatant in group D (two-step, thawing in water at 39°C) were significantly higher than those in any other group. Moreover, the ultrastructural alteration of oocyte in group D (two-step, thawing in water at 39°C) was slighter than those in any other group.
Conclusions:
The two-step protocol involving short-term exposure to water at a moderately high temperature (39°C) proved to be a suitable for thawing bovine whole ovaries.
