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The CHEVI tethering complex: facilitating special deliveries

Clare Rogerson1,2, Paul Gissen3,4,5

  • 1MRC Laboratory for Molecular Cell Biology, University College London, London, UK.

The Journal of Pathology
|August 25, 2016
PubMed

Insights

The CHEVI complex, comprising VPS33B and VIPAR, plays a role in endosomal trafficking. New research shows it

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Interactions

Background:

  • The class C Homologues in Endosome-Vesicle Interaction (CHEVI) complex, composed of VPS33B and VIPAR, is a recently identified tethering complex involved in endosomal trafficking.
  • Its precise function, particularly in specialized cellular processes like megakaryocyte α-granule biogenesis, remains under investigation, distinct from known HOPS and CORVET complexes.

Purpose of the Study:

  • To investigate the role of the CHEVI complex in α-granule biogenesis within megakaryocytes.
  • To identify novel interactors of the CHEVI complex and elucidate their functional significance.
  • To determine the requirement of VPS33B for the localization of specific proteins and cargo within megakaryocytes.

Main Methods:

  • Immunofluorescence microscopy to visualize protein localization.
  • Co-immunoprecipitation assays to identify protein interactions.
  • Analysis of megakaryocyte proplatelets to assess cargo transport.

Main Results:

  • The study identified α-tubulin and SEC22B as novel interactors of the CHEVI complex.
  • VPS33B expression was found to be essential for the correct localization of SEC22B and von Willebrand factor (VWF) to proplatelets.
  • These findings highlight the CHEVI complex's involvement in the specialized delivery of α-granule cargo.

Conclusions:

  • The CHEVI complex, through VPS33B, plays a critical role in the biogenesis of α-granules in megakaryocytes.
  • VPS33B mediates the localization of SEC22B and VWF, crucial for platelet formation.
  • This research expands our understanding of the CHEVI complex's function in cargo trafficking across different cell types.

Keywords:
CHEVI complexVIPARVPS33Bmegakaryocyteplatelettethering complexesvesicle traffickingα-granules

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