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Related Experiment Video

Updated: Mar 15, 2026

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia
07:48

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia

Published on: June 8, 2019

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Liposomal angiogenic peptides for ischemic limb perfusion: comparative study between different administration

Hyosook Hwang1, Hyeon-Soo Kim1, Hwan-Seok Jeong1

  • 1a Department of Nuclear Medicine , Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Sciences, Biomedical Research Institute, Research Institute of Clinical Medicine, Chonbuk National University Medical School and Hospital , Jeonju , South Korea.

Drug Delivery
|August 27, 2016
PubMed
Summary

PEGylated liposomes with angiogenic peptides effectively treat hindlimb ischemia by improving blood flow and promoting new vessel growth. Both intra-arterial and intramuscular delivery methods showed similar therapeutic benefits.

Keywords:
Liposomesischemiananoparticlespeptidestherapy

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Methods for Acute and Subacute Murine Hindlimb Ischemia
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Last Updated: Mar 15, 2026

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Methods for Acute and Subacute Murine Hindlimb Ischemia
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Methods for Acute and Subacute Murine Hindlimb Ischemia

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Vascular Biology

Background:

  • Peripheral limb ischemia is a condition characterized by reduced blood flow to the extremities, often leading to severe pain and tissue damage.
  • Current treatments for peripheral limb ischemia have limitations, necessitating the development of novel therapeutic strategies.
  • Angiogenic peptides hold promise for promoting new blood vessel formation, but their effective delivery remains a challenge.

Purpose of the Study:

  • To evaluate the therapeutic efficacy of PEGylated liposomes loaded with angiogenic peptides in a rat model of hindlimb ischemia.
  • To compare the effectiveness of intra-arterial infusion versus intra-muscular injection of these liposomal formulations.
  • To investigate the impact of this treatment on limb perfusion and the expression of angiogenic markers.

Main Methods:

  • Hindlimb ischemia was induced in rats via femoral artery occlusion.
  • PEGylated liposomes encapsulating angiogenic peptides were administered intra-arterially.
  • Limb perfusion was assessed using technetium-99m labeled red blood cell imaging before and after treatment.
  • Expression of angiogenic proteins, such as CD31, was analyzed post-treatment.
  • Therapeutic effects of intra-arterial versus intra-muscular administration were compared.

Main Results:

  • Femoral artery occlusion significantly reduced ischemic limb perfusion by approximately 69%.
  • Treatment with PEGylated liposomes loaded with angiogenic peptides significantly improved ischemic limb perfusion (210% of baseline) and increased CD31 expression (1.6-fold).
  • Neither liposomes nor peptides alone demonstrated significant therapeutic effects on perfusion or angiogenic response.
  • Intra-muscular injection of the liposomal formulation yielded comparable therapeutic effects on limb perfusion (230% of baseline) to intra-arterial infusion (210% of baseline).

Conclusions:

  • PEGylated liposomes loaded with angiogenic peptides represent a promising therapeutic strategy for peripheral limb ischemia.
  • The treatment effectively enhances limb perfusion and promotes angiogenesis.
  • Both intra-arterial infusion and intra-muscular injection are viable administration routes, offering similar therapeutic outcomes.