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Updated: Mar 15, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
Published on: April 26, 2013
DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization
Claudia Addamiano1, Béatrice Gerland2, Corinne Payrastre3
1Laboratoire de Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique, UMR CNRS 5068, Université Paul Sabatier, 118 Route de Narbonne, Cedex 9, Toulouse 31062, France. addamiano@chimie.ups-tlse.fr.
Abstract:
Construction and physico-chemical behavior of DNA three way junction (3WJ) functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 5'-C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl-, hydroxyl- or carboxyl-azide. Structural impacts of 5'-C(S)-functionalization were investigated to evaluate how 3WJ flexibility/stability is affected.
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