Related Experiment Video
Updated: Mar 15, 2026
An Ex vivo Culture System to Study Thyroid Development
Published on: June 6, 2014
Proteomic analysis reveals aberrant expression of CALR and HSPA5 in thyroid tissues of Graves' disease
Shuai Meng1, Wen Zhang1, Li-Juan Guan1
1Endocrinology Department, Jinshan Hospital of Fudan University, Shanghai 201508, China.
Objectives:
To explore the proteomic changes in thyroid tissue from GD patients and find new biomarkers for the prevention, diagnosis as well as the treatment of GD.
Design And Methods:
Group1 included five thyroid specimens of GD cases and 5 normal thyroid tissue samples which were removed surgically and collected. The proteins were extracted from these thyroid tissues and then the differentially expressed protein spots were identified by MALDI-TOF-MS. The interested proteins were further validated in more specimens (group2: 11 pathological thyroid specimens and 7 normal thyroid tissue samples).
Results:
A total of 34 differentially expressed proteins were observed, and the majority of these proteins were involved in endoplasmic reticulum stress (ER-stress), oxidative stress, energy metabolism, cytoskeleton and movement. The overexpression of calreticulin(CALR) and heat shock 70kDa protein 5(HSPA5) was further validated.
Conclusion:
Alltogether, abundant new candidate molecules, especially proteins related to ER-stress, were found to be involved in the pathogenesis of GD.
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