Related Experiment Video
Updated: Mar 15, 2026
A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
Published on: November 6, 2020
[Virulence of Sporothrix globosa in murine models]
Rodrigo Cruz Choappa1, Salomón Pérez Gaete2, Valentina Rodríguez Badilla2
1Cátedra de Micología, Universidad de Valparaíso, Unidad de Infectología, Hospital Carlos van Buren, Valparaíso, Chile.
Introduction:
The sporothricosis disease is an infection caused by species included in Sporothrix schenkii complex.
Objective:
Verify the virulence of a strain of S. globosa using two different concentrations of inoculum by intraperitoneally and subcutaneously, into a mouse model.
Materials And Methods:
Nonrandomized pilot study, in murine inoculated with a strain of S. globosa (CBS 14.076M) by intraperitoneally and subcutaneously with inoculum concentrations of 0.5 and 4 McFarland. For this purpose 18 rodents CF-1 (ISP, Santiago, Chile) were used.
Results:
The studied strain did not induce illness or injury on animals, they all survived and neither the tissue culture nor the histopathological analysis showed fungal growth or suggestive infection by organ abnormalities.
Conclusions:
The S. globosa strain did not present any virulence enough to cause disease at 0.5 and 4.0 McFarland concentration inoculum when inoculated in both intraperitoneally and subcutaneously, in murine models.
Insights
This study found that a specific strain of Sporothrix globosa did not cause illness in mice, even at higher concentrations. The fungus showed no virulence when injected intraperitoneally or subcutaneously.
Area of Science:
- Mycology
- Infectious Diseases
- Animal Models
Background:
- Sporothricosis is an infection caused by the Sporothrix schenkii complex.
- Understanding the virulence of different Sporothrix species is crucial for disease management.
Purpose of the Study:
- To evaluate the virulence of a Sporothrix globosa strain.
- To assess the impact of two different inoculum concentrations (0.5 and 4 McFarland).
- To determine virulence via intraperitoneal and subcutaneous inoculation in a mouse model.
Main Methods:
- A nonrandomized pilot study was conducted using 18 CF-1 mice.
- Mice were inoculated with Sporothrix globosa (CBS 14.076M) at 0.5 and 4 McFarland concentrations.
- Inoculations were administered via intraperitoneal and subcutaneous routes.
Main Results:
- All mice survived the inoculation with the S. globosa strain.
- No signs of illness or injury were observed in the animals.
- Histopathological analysis and tissue cultures did not reveal fungal growth or organ abnormalities indicative of infection.
Conclusions:
- The tested strain of Sporothrix globosa demonstrated no significant virulence.
- The fungus did not cause disease at the tested concentrations (0.5 and 4 McFarland) via intraperitoneal or subcutaneous inoculation in mice.
