Functionalized poly (ionic liquid) as the support to construct a ratiometric electrochemical biosensor for the...

An efficient ratiometric electrochemical biosensor for Cu²⁺ determination was constructed using dual hydroxyl-functionalized poly (ionic liquid) (DHF-PIL) as the catalyst support. The DHF-PIL exhibited typical macroporous structure, which provided a high surface area of 39.31m²/g for the sufficient loading of biomolecules. The specific recognition of Cu²⁺ was accomplished by employing neurokinin B (NKB) for the first time, which could bind to Cu²⁺ to form a [Cu^II(NKB)₂] complex with high specificity. Meanwhile, a common redox mediator, 2, 2'-Azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) was modified into DHF-PIL by electrostatic interactions to act as an inner reference molecule, which provided a built-in correction for environmental effects and improving the detection accuracy. With this strategy, the developed electrochemical biosensor was capable of determining Cu²⁺ with a linear range between 0.9 and 36.1μM and low detection limit (LOD) and quantification limit (LOQ) of 0.24 and 0.6μM, respectively. The sensor also displayed a satisfactory selectivity against a series of interferences in the brain, including metal ions, amino acids and other endogenous compounds. Accordingly, the present biosensor was successfully applied to evaluate Cu²⁺ levels in normal and AD rats.