Related Experiment Video
Updated: Mar 15, 2026

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
Published on: October 12, 2018
Combination of Raman Spectroscopy and Mass Spectrometry for Online Chemical Analysis
Anil Kumar Meher1, Yu-Chie Chen1
1Department of Applied Chemistry, National Chiao Tung University , Hsinchu 300, Taiwan.
Abstract:
Mass spectrometry (MS) and Raman spectroscopy are complementary analytical techniques used to provide information related to chemical structures and functional groups of target analytes. Each instrument provides specific chemical information. If these two analytical tools are coupled online, comprehensive structural information can be simultaneously collected from the analytes of interest without losing any important chemical information. Nevertheless, exploring a suitable interface for coupling of these analytical tools, which are governed with different operation principles, remains challenging. In this study, we used a small piece of tissue paper as an interface for hyphenating a Raman spectroscope and a mass spectrometer online. The paper played multiroles as sample loading substrate and an emitter to generate electrospray. Furthermore, it can facilitate surface-enhanced Raman spectroscopic analysis to improve analyte signals in Raman spectra. A sample droplet was placed on the tissue paper located close to the laser of the Raman spectroscope and the inlet of mass spectrometer. Raman spectra were first collected by the Raman spectroscope through laser irradiation followed by generation of electrospray on the edge of the paper for MS analysis. Positional isomers were used as model samples to demonstrate the effectiveness of the hyphenated analytical tool in distinguishing isomers. The feasibility of using this Raman-MS hyphenated technique for monitoring chemical reactions online in real time was also investigated.
More Related Videos
07:37An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
Published on: January 9, 2020
09:34Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Published on: September 20, 2016
Related Concept Videos
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Tandem Mass Spectrometry
Mass Spectrometry: Overview
MALDI-TOF Mass Spectrometry
Mass Spectrometers
High-Resolution Mass Spectrometry (HRMS)