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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Next-generation Sequencing03:00

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

Updated: Mar 15, 2026

DNA Stable-Isotope Probing DNA-SIP
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DNA Stable-Isotope Probing DNA-SIP

Published on: August 2, 2010

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Stable isotope labeling methods for DNA.

Frank H T Nelissen1, Marco Tessari1, Sybren S Wijmenga1

  • 1Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands.

Progress in Nuclear Magnetic Resonance Spectroscopy
|August 31, 2016
PubMed
Summary
This summary is machine-generated.

Nuclear Magnetic Resonance (NMR) is key for studying DNA. This review details isotope labeling strategies for stable isotope labeled DNA and building blocks, crucial for advanced NMR applications.

Keywords:
DNA synthesisLabeling methodsNMRNucleic acidsNucleotidesSite-specific

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Area of Science:

  • Biophysical Chemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful technique for analyzing biomolecules in solution.
  • Studying nucleic acids, particularly DNA, using NMR presents significant challenges compared to proteins due to inherent limitations in building blocks and spectral characteristics.
  • Site-specific isotope enrichment is essential for enabling advanced NMR experiments and applications with DNA molecules.

Purpose of the Study:

  • To provide a comprehensive review of isotope-labeling strategies for DNA.
  • To cover methods for obtaining stable isotope labeled DNA.
  • To discuss strategies for synthesizing specifically stable isotope labeled DNA building blocks for enzymatic synthesis.

Main Methods:

  • Review of existing literature on isotope enrichment techniques for DNA.
  • Analysis of strategies for site-specific and general stable isotope labeling of DNA.
  • Examination of methods for preparing isotopically labeled nucleoside triphosphates for enzymatic DNA synthesis.

Main Results:

  • Detailed overview of various stable isotope labeling approaches for DNA.
  • Identification of key challenges and solutions in achieving site-specific isotope labeling.
  • Compilation of methods for producing labeled DNA building blocks suitable for enzymatic polymerization.

Conclusions:

  • Isotope labeling is indispensable for high-resolution NMR studies of DNA structure and dynamics.
  • Effective labeling strategies enable the application of advanced NMR techniques to complex DNA systems.
  • The reviewed methods facilitate the production of custom-labeled DNA for diverse research applications.