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Updated: May 31, 2025
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Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Published on: December 27, 2016
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Hyphenation of 2D NMR With Hydrogenative PHIP
Bono O Jimmink1, Marco Tessari1, Arno P M Kentgens1
1Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands.
Magnetic Resonance in Chemistry : MRC
|January 22, 2025
Summary
Parahydrogen induced polarization (PHIP) enhances NMR sensitivity. New 2D NMR methods spread hyperpolarized signals across molecules, enabling faster, more sensitive analysis at lower concentrations.
Area of Science:
- Nuclear Magnetic Resonance Spectroscopy
- Hyperpolarization Techniques
- Organic Chemistry
Background:
- Parahydrogen induced polarization (PHIP) significantly enhances Nuclear Magnetic Resonance (NMR) sensitivity.
- Hyperpolarization in PHIP is typically confined to protons directly involved in the addition reaction.
- Existing pulse sequences can distribute this hyperpolarization throughout the molecule's coupled proton network.
Purpose of the Study:
- To extend the reach of PHIP by applying 2D NMR spectroscopy to the hyperpolarized proton network.
- To enable faster and more sensitive molecular analysis by acquiring information from the entire molecule.
- To demonstrate a novel approach for enhanced NMR detection at low concentrations.
Main Methods:
- Implementation of 2D NMR spectroscopy during parahydrogenation.
- Utilizing pulse schemes to spread hyperpolarized magnetization across J-coupled protons.
- Performing a 2D Total Correlation Spectroscopy (TOCSY) experiment during hydrogenative PHIP.
Main Results:
- Successfully acquired a 2D NMR spectrum within minutes.
- Demonstrated the method's efficacy at micromolar concentrations.
- Obtained comprehensive molecular information from the entire hyperpolarized network.
Conclusions:
- The novel 2D NMR approach effectively expands the utility of PHIP.
- This methodology allows for rapid and highly sensitive characterization of molecules at low concentrations.
- The technique shows significant promise for broader applications in chemical analysis and research.
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