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Understanding how mismatch repair proteins participate in the repair/anti-recombination decision

Ujani Chakraborty1, Eric Alani2

  • 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703, USA.

FEMS Yeast Research
|August 31, 2016
PubMed

Insights

DNA mismatch repair (MMR) systems and anti-recombination pathways resolve DNA sequence variations. Specific factors regulate the choice between these pathways, influencing genetic stability.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA mismatch repair (MMR) corrects errors during DNA replication.
  • Recombination can create heteroduplex DNA with mismatches.
  • Heteroduplexes can be resolved by MMR or anti-recombination (heteroduplex rejection).

Purpose of the Study:

  • To review factors regulating the MMR/anti-recombination decision.
  • To present models for how this decision is modulated.

Main Methods:

  • Literature review of studies, primarily in baker's yeast.
  • Analysis of recent advances in understanding DNA repair and recombination pathways.

Main Results:

  • Specific factors influence the choice between MMR and anti-recombination.
  • DNA structure, protein levels and localization, and epigenetic marks modulate this decision.

Conclusions:

  • The MMR/anti-recombination decision is a critical regulatory process.
  • Multiple factors interact to control DNA sequence fidelity during replication and recombination.
Keywords:
DNA mismatch repairMsh2Msh3Msh6chromatin marksheteroduplex rejection

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