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In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
Published on: May 1, 2020
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iTRAQ-based proteomic profiling reveals different protein expression between normal skin and hypertrophic scar tissue
Jianglin Tan1, Weifeng He1, Gaoxing Luo1
1Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injuries, Chongqing Key Laboratory for Disease Proteomics, Southwest Hospital, Third Military Medical University, Chongqing, 400038 China.
Burns & Trauma
|August 31, 2016
Summary
This study identified 89 differential proteins in hypertrophic scars using iTRAQ technology. These protein changes in scar tissue may reveal new therapeutic targets for this fibrotic condition.
Area of Science:
- Biochemistry
- Proteomics
- Dermatology
Background:
- Hypertrophic scars are a challenging human fibrotic condition.
- Current treatments for hypertrophic scars remain limited.
- Understanding scar formation mechanisms is crucial for effective management.
Purpose of the Study:
- To investigate the molecular mechanisms of hypertrophic scar formation.
- To identify differential protein expression in hypertrophic scars compared to normal skin.
- To explore potential therapeutic targets for hypertrophic scar treatment.
Main Methods:
- Utilized isobaric tags for relative and absolute quantitation (iTRAQ) labeling.
- Employed high-throughput 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS).
- Analyzed differential protein expression between hypertrophic scar and normal skin tissues.
Main Results:
- Identified 3166 proteins with high confidence.
- Detected 89 differentially expressed proteins between hypertrophic scars and normal skin.
- Found 41 up-regulated proteins (extracellular matrix related) and 48 down-regulated proteins (junction/structural molecule activity related).
Conclusions:
- Demonstrated 89 differentially expressed proteins in hypertrophic scars via iTRAQ.
- These proteins may elucidate the pathology of hypertrophic scar formation.
- Findings could inform novel therapeutic strategies for hypertrophic scars.
