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Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes
Joanne A Hammill1, Arya Afsahi1, Jonathan L Bramson2
1Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5.
Abstract:
The adoptive transfer of a bolus of tumor-specific T lymphocytes into cancer patients is a promising therapeutic strategy. In one approach, tumor specificity is conferred upon T cells via engineering expression of exogenous receptors, such as chimeric antigen receptors (CARs). Here, we describe the generation and production of both murine and human CAR-engineered T lymphocytes using retroviruses.
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