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Updated: Mar 15, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

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RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae

Tong Si1, Huimin Zhao2,3,4

  • 1Carl R Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2016
PubMed
Summary

RNA interference-assisted genome evolution (RAGE) uses directed evolution to engineer yeast. This method iteratively improves traits like acetic acid tolerance by accumulating genetic modifications for enhanced yeast genome evolution.

Keywords:
Directed evolutionGenome engineeringHigh-throughput screeningInhibitor toleranceRNA interferenceSaccharomyces cerevisiaeSynthetic biology

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