Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Excision Repair-Initiated Enzyme-Assisted Bicyclic Cascade Signal Amplification for Ultrasensitive Detection of Uracil-DNA Glycosylase.

Analytical chemistry·2017
Same author

Development of fluorescent methods for DNA methyltransferase assay.

Methods and applications in fluorescence·2017
Same author

Waist-hip Ratio (WHR), a Better Predictor for Prostate Cancer than Body Mass Index (BMI): Results from a Chinese Hospital-based Biopsy Cohort.

Scientific reports·2017
Same author

Chronic nicotine differentially affects murine transcriptome profiling in isolated cortical interneurons and pyramidal neurons.

BMC genomics·2017
Same author

Combinatorial Strategy to Identify Fluorescent Probes for Biothiol and Thiophenol Based on Diversified Pyrimidine Moieties and Their Biological Applications.

Analytical chemistry·2017
Same author

Highly Specific and Ultrasensitive Two-Photon Fluorescence Imaging of Native HOCl in Lysosomes and Tissues Based on Thiocarbamate Derivatives.

Analytical chemistry·2017
Same journal

Design Principles for Negative Thermal Expansion in Two-Dimensional Materials.

Accounts of chemical research·2026
Same journal

Main Group Redox Catalysis: New Frontiers with Germanium and Tin.

Accounts of chemical research·2026
Same journal

Taming Irreversibility in sp<sup>2</sup>-Carbon-Conjugated COFs from Polycrystalline Powders to Single Crystals and Thin Films.

Accounts of chemical research·2026
Same journal

Electroactive Imidazolium Ionic Liquids in Organic Synthesis.

Accounts of chemical research·2026
Same journal

Calix[4]resorcinarene-Based Porous Organic Cages: Synthesis and Applications.

Accounts of chemical research·2026
Same journal

Light-Driven Dual Rotary Molecular Motors and Beyond.

Accounts of chemical research·2026
See all related articles

Related Experiment Video

Updated: Mar 15, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.8K

Fluorescent Biosensors Based on Single-Molecule Counting.

Fei Ma1, Ying Li2, Bo Tang1

  • 1College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University , Jinan 250014, China.

Accounts of Chemical Research
|September 2, 2016
PubMed
Summary
This summary is machine-generated.

Single-molecule counting biosensors offer ultrasensitive detection of biomolecules for diagnostics. These fluorescent sensors provide rapid, selective, and simple quantification, overcoming limitations of conventional methods.

More Related Videos

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.3K
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.6K

Related Experiment Videos

Last Updated: Mar 15, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.8K
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.3K
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.6K

Area of Science:

  • Biomedical research and molecular diagnostics
  • Development of ultrasensitive biosensors

Background:

  • Conventional biomolecular assays lack sensitivity and specificity for low-concentration targets.
  • Urgent need for rapid, ultrasensitive analytical tools in areas like early disease diagnostics.
  • Single-molecule fluorescence approaches show promise for overcoming these limitations.

Purpose of the Study:

  • To summarize efforts in developing ultrasensitive biosensors based on single-molecule counting.
  • To demonstrate the application of these biosensors for detecting various disease-related biomarkers.

Main Methods:

  • Utilizing single-molecule counting, a technique that quantifies target molecules by counting individual fluorescent bursts.
  • Employing specific in vitro/in vivo fluorescent labeling to translate biomolecule presence into fluorescence signals.
  • Detection via microfluidic device-integrated confocal microscopy or total internal reflection fluorescence-based single-molecule imaging.

Main Results:

  • Development of a series of single-molecule counting-based biosensors, including separation-free and separation-assisted assays.
  • Successful proof-of-concept demonstration for sensitive detection of DNAs, miRNAs, proteins, enzymes, and intact cells.
  • These detected biomolecules can serve as disease-related biomarkers.

Conclusions:

  • Single-molecule counting biosensors offer ultrahigh sensitivity, good selectivity, rapid analysis, and low sample consumption.
  • Future directions include developing user-friendly instruments, new fluorescent labels, and novel biosensor designs.
  • These biosensors hold significant potential for biological research, clinical diagnostics, and drug discovery.