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Updated: Mar 15, 2026
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
Published on: August 23, 2019
In vitro analysis of partially hydrolyzed guar gum fermentation on identified gut microbiota
Justin Carlson1, Trevor Gould2, Joanne Slavin1
1Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave St. Paul, MN 55108, United States.
Background:
Prebiotic dietary fibers resist digestion in the upper gastrointestinal tract and allow for stimulation of bacteria in the distal intestine and colon. Stimulation of bacteria among different individuals varies greatly, depending on a wide range of variables.
Objective:
To determine the range of differences in response between individuals, a preclinical in vitro fermentation was conducted with six fecal donors. The primary objective was to compare the fecal microbiota of six individuals at baseline, 12 h and 24 h post-exposure to partially hydrolyzed guar gum (PHGG).
Method:
Fecal donations were collected from six healthy individuals consuming a non-specific Western diet, free of antibiotic treatments in the past year, not affected by any GI diseases and not consuming any probiotic or prebiotic supplements. Fecal samples were exposed to 0.5 g of PHGG and measured for bacterial changes at 0, 12 and 24 h based on 16S rRNA sequencing.
Results:
Parabacteroides increased from 3.48% of sequence reads to 10.62% of sequence reads after 24 h (p = 0.0181) and Bacteroidetes increased from 45.89% of sequence reads to 50.29% of sequence reads (p = 0.0008).
Conclusions:
PHGG stimulates growth of Parabacteroides, a genus of bacteria that have been inversely associated with IBS and ulcerative colitis. PHGG provides stimulation of beneficial Bacteroidetes (Bacteroides and Parabacteroides), which may be correlated with many positive health markers and outcomes. PHGG is a prebiotic dietary fiber that is readily fermentable.
