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A Long Noncoding RNA ZEB1-AS1 Promotes Tumorigenesis and Predicts Poor Prognosis in Glioma
Qiao-Li Lv1,2, Lei Hu3,4, Shu-Hui Chen5
1Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. lvqiaoli2008@126.com.
International Journal of Molecular Sciences
|September 3, 2016
Summary
Long noncoding RNA ZEB1 antisense 1 (ZEB1-AS1) is highly expressed in glioma and linked to poor prognosis. Silencing ZEB1-AS1 inhibits glioma cell growth and metastasis, suggesting it as a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
- The specific role and mechanism of lncRNA ZEB1 antisense 1 (ZEB1-AS1) in glioma remain largely unknown.
Purpose of the Study:
- To investigate the clinical significance, biological function, and molecular mechanisms of ZEB1-AS1 in glioma.
- To evaluate ZEB1-AS1 as a potential prognostic biomarker and therapeutic target for glioma.
Main Methods:
- Quantitative real-time PCR to assess ZEB1-AS1 expression in glioma tissues.
- Cell proliferation, migration, invasion, and apoptosis assays following ZEB1-AS1 knockdown.
- Cell cycle analysis and Western blotting to elucidate molecular pathways involved.
Main Results:
- ZEB1-AS1 expression was significantly upregulated in glioma tissues and correlated with advanced clinical stage.
- High ZEB1-AS1 levels predicted poor prognosis, independent of other clinical factors.
- ZEB1-AS1 knockdown inhibited cell proliferation, migration, and invasion, induced G0/G1 cell cycle arrest, and promoted apoptosis.
- ZEB1-AS1 regulates the cell cycle via Cyclin D1 and CDK2, and promotes epithelial-to-mesenchymal transition (EMT) by modulating key EMT markers and matrix metalloproteinases.
Conclusions:
- ZEB1-AS1 is a potential independent prognostic biomarker for glioma.
- ZEB1-AS1 plays a critical role in glioma progression, acting as a regulator of cell cycle, proliferation, migration, invasion, and apoptosis.
- ZEB1-AS1 represents a promising therapeutic target for glioma treatment.
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