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Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

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Related Experiment Video

Updated: Mar 15, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Visualizing Wnt Palmitoylation in Single Cells.

Xinxin Gao¹, Rami N Hannoush²

¹Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390-9039, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 4, 2016

Summary

This summary is machine-generated.

We developed a sensitive method to detect Wnt palmitoylation, a key process in Wnt protein secretion and signaling. This technique uses bio-orthogonal probes and proximity ligation assay (PLA) for high-resolution imaging.

Keywords:

Alkyne fatty acid Click chemistry Microscopy Proximity ligation Wnt palmitoylation

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Last Updated: Mar 15, 2026

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Published on: December 10, 2021

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Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Wnt palmitoylation is crucial for regulating Wnt protein secretion and cellular signaling.
Monitoring Wnt palmitoylation is essential for understanding Wnt pathway activity and membrane interactions.

Purpose of the Study:

To describe a novel, highly sensitive method for detecting cellular Wnt palmitoylation.
To enable detailed investigation of Wnt protein modification and localization.

Main Methods:

The method combines click chemistry with bio-orthogonal fatty acid probes.
Proximity Ligation Assay (PLA) is utilized for high-sensitivity detection.
The protocol is compatible with various imaging platforms.

Main Results:

The developed method offers high sensitivity and subcellular resolution for Wnt palmitoylation detection.
It allows for visualization of Wnt palmitoylation within cellular compartments.
The technique is adaptable for studying other fatty acylated proteins.

Conclusions:

This protocol provides a powerful tool for studying Wnt palmitoylation.
It facilitates research into Wnt secretion, signaling, and protein acylation.
The method's versatility extends to other acylated proteins.