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Related Experiment Video

Updated: Mar 15, 2026

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

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Published on: December 14, 2015

Biochemical Methods to Analyze Wnt Protein Secretion.

Kathrin Glaeser¹, Michael Boutros¹, Julia Christina Gross²

¹Division Signaling and Functional Genomics and Heidelberg University, Department for Cell and Molecular Biology, German Cancer Research Center (DKFZ), Medical Faculty Mannheim, Im Neuenheimer Feld 580, 69120, Heidelberg, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|September 4, 2016

Summary

This study introduces methods to purify and quantify Wnt proteins in cell culture supernatants. These techniques help researchers understand Wnt secretion pathways and their role in development and cancer.

Keywords:

Blue Sepharose Hydrophobic proteins Purified Wnt Wnt secretion Wnt signaling activity Wnts on extracellular vesicles

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Last Updated: Mar 15, 2026

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Published on: December 14, 2015

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Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

Wnt proteins are crucial morphogens in development and homeostasis.
Dysregulated Wnt signaling is implicated in various cancers.
Wnt secretion involves complex intracellular trafficking, but pathways remain unclear.

Purpose of the Study:

To describe protocols for purifying and quantifying Wnt proteins from cell supernatants.
To enable investigation of Wnt protein secretion pathways and extracellular modulators.

Main Methods:

Purification of Wnt proteins from cell culture supernatant.
Quantification of total Wnt proteins via immunoblotting.
Assessment of canonical Wnt activity using a dual-luciferase reporter assay.

Main Results:

Established protocols for isolating and measuring Wnt proteins in supernatant.
Enabled simultaneous assessment of Wnt protein levels and biological activity.
Facilitated the study of Wnt protein trafficking and secretion mechanisms.

Conclusions:

The described methods allow for detailed analysis of Wnt secretion.
These protocols are valuable tools for studying Wnt-related biological processes and diseases.
Understanding Wnt secretion is key to targeting Wnt signaling in cancer.