Related Experiment Video
Updated: Mar 15, 2026
08:25
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
23.2K
Tetrazine-based chemistry for nitrite determination in a paper microfluidic device
Inmaculada Ortiz-Gomez1, Mariano Ortega-Muñoz2, Alfonso Salinas-Castillo1
1Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, 18071, Spain.
Talanta
|September 4, 2016
Summary
A new microfluidic paper analytical device (µPAD) offers a simple method for nitrite determination. This colorimetric approach using s-dihydrotetrazine (DHBPTz) and smartphone analysis provides accurate nitrite quantification in water samples.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Materials Science
Background:
- Nitrite detection is crucial for water quality assessment.
- Existing methods can be complex or require specialized equipment.
- Microfluidic paper-based analytical devices (µPADs) offer portable and cost-effective solutions.
Purpose of the Study:
- To develop and validate a novel µPAD for sensitive and selective nitrite determination.
- To utilize smartphone-based image analysis for quantitative nitrite detection.
- To assess the method's performance and applicability to real-world water samples.
Main Methods:
- Fabrication of a cellulose paper-based µPAD with immobilized recognition chemistry.
- Colorimetric detection based on the oxidation of s-dihydrotetrazine (DHBPTz) by nitrite-derived nitrous acid.
- Quantitative analysis using smartphone imaging and the S-coordinate of the HSV color space.
Main Results:
- The method achieved a detection limit of 1.30µM for nitrite.
- Optimization of reagent concentrations, pH, sample volume, and reaction time was performed.
- Interference studies indicated good selectivity for nitrite in water samples.
Conclusions:
- The developed µPAD provides a simple, rapid, and portable method for nitrite analysis.
- Smartphone-based quantification offers a user-friendly approach for field applications.
- The method demonstrates potential for routine monitoring of nitrite in natural water sources.
