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Protein Dynamics in Living Cells

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Activatable fluorescence: From small molecule to nanoparticle.

Benjamin M Luby¹, Danielle M Charron², Christina M MacLaughlin¹

¹Princess Margaret Cancer Centre and Techna Institute, University Health Network, Toronto, ON, Canada.

Advanced Drug Delivery Reviews

|September 6, 2016

Summary

This summary is machine-generated.

Activatable fluorescence imaging agents are crucial for drug delivery systems, offering real-time insights into biological targets. Their design, from small molecules to nanomaterials, depends on the specific application and target complexity.

Keywords:

Aggregation Beacon Biosensing Fluorophore Molecular imaging Nanocarrier Smart probe Theranostics

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Area of Science:

Biomedical Engineering
Molecular Imaging
Nanotechnology

Background:

Molecular imaging is vital for developing and applying drug delivery systems.
Fluorescence imaging provides signal specificity and dynamic information.
Activatable fluorescence imaging leverages agent responsivity to biological targets for fluorogenic responses.

Purpose of the Study:

To review the principles and applications of activatable fluorescence imaging agents in drug delivery.
To discuss the design considerations for different scales of activatable agents (small molecule, macromolecular, nanoscale).
To highlight the importance of matching agent design to molecular imaging applications and biological target complexity.

Main Methods:

Review of literature on activatable fluorescence imaging agents.
Analysis of agent design strategies based on size scale and activation mechanisms.
Discussion of the advantages and challenges of different agent types for molecular imaging.

Main Results:

Activatable fluorescence agents can be designed as small molecules, macromolecules, or nanomaterials.
Nanoscale agents combine fluorophore responsivity with nanomaterial properties.
Agent selection depends on target characteristics and imaging goals.

Conclusions:

Activatable fluorescence imaging is a powerful tool for drug delivery system development.
The choice of agent size and activation strategy is critical for successful molecular imaging.
Future advancements will likely focus on sophisticated nanoscale activatable agents.