Related Experiment Video

Updated: Mar 15, 2026

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

3.2K

Fbxw7β, E3 ubiquitin ligase, negative regulation of primary myoblast differentiation, proliferation and migration

Kyungshin Shin1,2, Sang-Gu Hwang1, Ik Joon Choi3

  • 1Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Science, Seoul, South Korea.

Animal Science Journal = Nihon Chikusan Gakkaiho
|September 6, 2016
PubMed

Insights

Fbxw7β negatively regulates skeletal muscle regeneration by controlling myoblast differentiation, proliferation, and migration. Inhibiting Fbxw7β may offer a therapeutic strategy for muscle diseases.

Area of Science:

  • Muscle biology
  • Cellular regeneration
  • Biochemistry

Background:

  • Satellite cells are crucial for skeletal muscle regeneration, involving proliferation, migration, and fusion.
  • The precise regulators of myoblast behavior during regeneration remain incompletely understood.

Purpose of the Study:

  • To investigate the role of Fbxw7 (F-box only protein 7) in primary myoblasts.
  • To assess Fbxw7's potential as a therapeutic target for muscle diseases.

Main Methods:

  • Evaluation of Fbxw7β function in primary myoblasts and satellite cells.
  • Analysis of myoblast differentiation, proliferation, and migration.
  • Utilizing a dominant-negative F-box deleted Fbxw7β mutant.

Main Results:

  • Fbxw7β was identified as a negative regulator of myoblast and satellite cell differentiation, proliferation, and migration.
  • These inhibitory effects were dependent on Fbxw7β's E3 ubiquitin ligase activity, as a mutant lacking the F-box domain did not elicit the same response.

Conclusions:

  • Fbxw7β plays a significant role in modulating myoblast behavior during muscle regeneration.
  • Targeting Fbxw7β presents a potential therapeutic avenue for enhancing muscle regeneration and treating muscle diseases.
Keywords:
Fbxw7βdifferentiationmigrationmyoblastskeletal muscle

More Related Videos

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

10.3K
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells09:39

Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

Published on: July 29, 2016

16.1K

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.8K
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
6.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.9K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.8K