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A method of human diaphorase locus 3 phenotyping in human semen by isoelectric focusing
1Institute of Forensic Medicine, Medical Academy Gdańsk, Poland.
Forensic Science International
|July 1, 1989
Abstract:
A rapid method for the separation of diaphorase (DIA 3) isozymes in the pH range 6-8 is presented. Isoelectric focusing (IEF) of DIA 3 on 0.2 mm thick polyacrylamide gels took 60 min at 5 degrees C. Separated bands are sharp and common phenotypes are readily distinguishable. The method combines high resolution, speed, reproducibility and reagent economy with simplicity.
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