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Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging.

Michelle Cua¹, Daniel J Wahl¹, Yuan Zhao¹

¹School of Engineering Science, Simon Fraser University, Burnaby, BC V5A 1S6 Canada.

Scientific Reports

|September 8, 2016

Summary

This summary is machine-generated.

Sensorless adaptive optics correct aberrations in scattering tissues for deeper multiphoton microscopy. This technique uses low-coherence interferometry for depth-resolved aberration correction, improving in vivo imaging of mouse retinas.

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Area of Science:

Biomedical Optics
Microscopy
Biophysics

Background:

Multiphoton microscopy allows deep tissue imaging but is limited by scattering.
Aberrations from refractive index variations distort focal spots, reducing signal efficiency.
Conventional adaptive optics struggle with thick, scattering biological samples.

Purpose of the Study:

To develop a sensorless adaptive optics method for depth-resolved aberration correction in scattering tissues.
To improve two-photon excited fluorescence (TPEF) imaging in biological samples.
To enable effective aberration correction in challenging in vivo imaging scenarios.

Main Methods:

Utilized sensorless adaptive optics (SAO) with low-coherence interferometric detection.
Employed a transmissive multi-actuator adaptive lens for wavefront correction.
Demonstrated coherence-gated SAO TPEF for aberration correction in biological tissue.

Main Results:

Achieved depth-resolved aberration correction in scattering biological tissue.
Successfully performed in vivo imaging of a mouse retina using the developed SAO TPEF system.
Showcased the potential for reduced laser power requirements in multiphoton imaging.

Conclusions:

Sensorless adaptive optics with low-coherence interferometry offers effective aberration correction for multiphoton microscopy in scattering tissues.
The demonstrated system enables improved in vivo imaging, particularly in sensitive tissues like the mouse retina.
This approach facilitates integration with existing multiphoton microscopy systems and reduces laser power demands.