A biological membrane-based novel excisional wound-splinting model in mice (With video)

Rodents have robust wound healing mechanism compared to other animal species. The major mechanisms of wound healing differ between rodents and humans. In humans, wound healing primarily depends on re-epithelialization and granulation tissue (GT) formation, whereas wound contraction is more important during rodent wound closure. In this study, we described a novel excisional wound-splinting model in mice with a new biological membrane to imitate wound healing in humans. In this model, wound contraction can be effectually prevented, and the extent of re-epithelialization and the amount of granulation tissue can be determined easily. Furthermore, the harvested tissues can be analyzed with different methods according to the research aim. In conclusion, we have developed a biological membrane-based, novel, excisional wound-splinting model in mice that has unique advantages for wound healing research compared with the conventional animal model.