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Updated: Mar 15, 2026

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

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Published on: December 12, 2013

Nanoscopic Cellular Imaging: Confinement Broadens Understanding.

Stephen A Lee¹, Aleks Ponjavic², Chanrith Siv¹

¹Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109, United States.

|September 8, 2016

Summary

This summary is machine-generated.

Single-molecule fluorescence imaging advances nanoscale visualization in living cells. This review explores three optical excitation confinement methods for improved subcellular biophysics resolution.

Keywords:

bacterial biophysics light-sheet microscopy nanophotonics optical sectioning plasmonic enhancement single-molecule imaging super-resolution microscopy

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Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

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Published on: December 14, 2009

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Published on: December 14, 2009

Area of Science:

Biophysics
Optical Microscopy
Cell Biology

Background:

Single-molecule fluorescence imaging bridges optical microscopy and subcellular biophysics.
Current limitations in nanoscale imaging of living cells necessitate advanced optical techniques.

Purpose of the Study:

To review methods for optical excitation confinement enabling nanoscale imaging in living cells.
To discuss the potential of these techniques for advancing subcellular biophysics.

Main Methods:

Review of excitation confinement via laser illumination and beam shaping.
Review of physical confinement using micron-scale geometries in bacteria.
Review of nanoscale confinement employing nanophotonics.

Main Results:

Identified three distinct confinement geometries for nanoscale imaging.
Highlighted the role of optical excitation confinement in overcoming current imaging barriers.
Discussed the application of these methods across different biological systems.

Conclusions:

Optical excitation confinement is crucial for next-generation nanoscale imaging in living cells.
These confinement strategies offer pathways to achieve higher resolution in subcellular biophysics.
The reviewed methods provide a foundation for future advancements in live-cell nanoscale imaging.