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Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans.

Tisha Bohr1, Guinevere Ashley1, Evan Eggleston1

  • 1Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, California 95064.

Genetics
|September 9, 2016
PubMed
Summary

The synapsis checkpoint ensures proper chromosome pairing during meiosis by monitoring synaptonemal complex (SC) assembly. This study reveals SC components are crucial for checkpoint function, suggesting it monitors SC assembly, not just homolog pairing.

Keywords:
checkpointchromosomemeiosissynapsissynaptonemal complex

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Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Synapsis, the pairing of homologous chromosomes, requires the synaptonemal complex (SC) for accurate meiotic segregation.
  • The Caenorhabditis elegans synapsis checkpoint eliminates cells with unsynapsed chromosomes via apoptosis.
  • This checkpoint relies on pairing centers (PCs) that facilitate chromosome pairing and synapsis.

Purpose of the Study:

  • To investigate the role of SC components in the synapsis checkpoint.
  • To test the hypothesis that the stability of homolog pairing at PCs is monitored by the checkpoint.
  • To determine if the synapsis checkpoint monitors SC assembly in addition to homolog pairing.

Main Methods:

  • Genetic analysis of synaptonemal complex (SC) components (SYP-3, HTP-3, HIM-3, HTP-1) in Caenorhabditis elegans.
  • Assessment of pairing center (PC) function and homolog pairing stability in mutant backgrounds.
  • Evaluation of the synapsis checkpoint response in relation to PC function and SC assembly.

Main Results:

  • SYP-3, HTP-3, HIM-3, and HTP-1 are essential for a functional synapsis checkpoint.
  • Mutations in these SC components do not abolish PC function, confirming their role as checkpoint components.
  • Instability of homolog pairing at PCs does not always correlate with the synapsis checkpoint response in certain mutant backgrounds.

Conclusions:

  • The synapsis checkpoint requires specific synaptonemal complex (SC) components for its function.
  • These findings suggest the synapsis checkpoint monitors not only the stability of homolog pairing but also the assembly of the SC.
  • This provides new insights into the regulatory mechanisms ensuring accurate meiotic chromosome segregation.