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Related Concept Videos

MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Performing Custom MicroRNA Microarray Experiments
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Published on: October 28, 2011

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Phenotypic MicroRNA Microarrays.

Yong-Jun Kwon1, Jin Yeong Heo2, Hi Chul Kim3

  • 1Institut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Korea. yjkwon@ip-korea.org.

Microarrays (Basel, Switzerland)
|September 9, 2016
PubMed
Summary

This study explores using microarray technology for high-throughput screening of microRNA libraries. MicroRNA transfection demonstrated comparable effects to siRNA on cellular phenotypes, including NF-κB regulation.

Keywords:
microRNAphenotypic screensiRNA

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Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Microarray technology enables high-throughput analysis for repeated experiments and diverse samples.
  • Current applications involve visualizing siRNA/cDNA effects on cellular morphology and phenotype via microscopy.
  • Investigating microRNAs (miRNAs) using these established high-density microarray techniques is a novel approach.

Purpose of the Study:

  • To explore the utility of micro-scale, high-throughput microarray technology for studying microRNA biology.
  • To assess the phenotypic impact of microRNAs in cellular models.
  • To evaluate microRNA libraries for high-content screening in disease models.

Main Methods:

  • Utilized high-density spots microarray for microscopy visualization.
  • Performed reverse-transfection of microRNAs and small interfering RNA (siRNA).
  • Analyzed cellular phenotype and NF-κB expression levels post-transfection.

Main Results:

  • MicroRNA transfection induced cellular phenotypes comparable to siRNA.
  • MicroRNAs were shown to regulate NF-κB expression.
  • The study successfully demonstrated the feasibility of using microarrays for miRNA studies.

Conclusions:

  • Microarray-based reverse-transfection is a viable method for high-throughput microRNA screening.
  • This approach facilitates phenotypic screening of microRNA libraries in cellular disease models.
  • The technology offers a promising avenue for understanding microRNA function in biological systems.