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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Mar 15, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

Integrated Amplification Microarrays for Infectious Disease Diagnostics.

Darrell P Chandler¹, Lexi Bryant², Sara B Griesemer³

¹Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA. dchandler@akonni.com.

Microarrays (Basel, Switzerland)

|September 9, 2016

Summary

This study presents integrated microarray biochemistry for improved diagnostic tests. This approach streamlines workflows, reduces contamination, and lowers costs for infectious disease diagnostics.

Keywords:

RT-PCR asymmetric PCR diagnostics gel element arrays integrated microarrays microfluidics multiplex reverse transcriptase

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Last Updated: Mar 15, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

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Published on: April 25, 2014

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

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Published on: June 9, 2011

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Area of Science:

Biotechnology
Molecular Diagnostics
Microfluidics

Background:

Microarray-based tests are crucial for diagnostics but can be complex and prone to contamination.
Current workflows often involve multiple steps, increasing processing time and risk of errors.
There is a need for streamlined, closed-system approaches to enhance microarray utility.

Purpose of the Study:

To describe an integrated microarray-based test combining amplification and hybridization in a single microfluidic chamber.
To demonstrate an approach that simplifies workflow and minimizes cross-contamination for diagnostic applications.
To highlight the potential for reduced complexity and cost in microarray technology.

Main Methods:

Integration of solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber.
Development of a closed-system consumable architecture incorporating wash steps.
Application of the integrated approach to DNA and RNA genomes.

Main Results:

The integrated biochemical approach significantly reduces the number of steps in microarray workflow.
Minimization of sample or amplicon cross-contamination is achieved.
A simple consumable architecture for a closed system is demonstrated.

Conclusions:

Integrated microarray biochemistry offers a streamlined workflow for diagnostic applications.
This approach has the potential to significantly reduce complexity and cost of microarray systems.
Wider adoption of multiplexed microarray diagnostics, particularly for infectious diseases, is anticipated.