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Optical coherence tomography analysis of hydrofluoric acid decontamination of human cornea by mannitol solution
Ricardo M Nosé1, Fabio B Daga2, Walton Nosé3
1Irmandade da Santa Casa de Misericordia de Sao Paulo, Sao Paulo, Brazil; Eye Clinic, Sao Paulo, Brazil.
Purpose:
To evaluate the efficacy of mannitol solution as a decontamination agent on the chemical burn of the human corneas.
Methods:
Eight donor corneas from an eye bank were exposed to 25μl of 2.5% hydrofluoric acid (HF) solution on a filter paper for 20s. Three eyes were rinsed with 1000ml of mannitol 20% for 15min immediately after removal of the filter paper, 3 other were rinsed with sodium chloride (NaCl) 0.9% (1000ml for 15min) and two eyes were not rinsed. Microstructural changes were monitored in the time domain by optical coherence tomography (OCT) imaging for 75min.
Results:
NaCl reduced the penetration depth to approximately half the thickness of the cornea at 15min; scattering within the anterior cornea was higher than that for the unrinsed eye. With mannitol, no increased scattering was observed in the posterior part of the corneal stroma within a time period of 1h after rinsing. OCT images revealed low-scattering intensity within the anterior stroma at the end of the rinsing period.
Conclusion:
In eye bank human corneas, mannitol proved to be an efficient agent to decontaminate HF burn.
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