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Identification of Gastric Cancer Biomarkers Using 1H Nuclear Magnetic Resonance Spectrometry
Gokula Krishnan Ramachandran1, Wei Peng Yong2, Chen Hua Yeow1
1Department of Biomedical Engineering, National University of Singapore, Singapore, Singapore.
Abstract:
Existing gastric cancer diagnosing methods were invasive, hence, a reliable non-invasive gastric cancer diagnosing method is needed. As a starting point, we used 1H NMR for identifying gastric cancer biomarkers using a panel of gastric cancer spheroids and normal gastric spheroids. We were able to identify 8 chemical shift biomarkers for gastric cancer spheroids. Our data suggests that the cancerous and non-cancerous spheroids significantly differ in the lipid composition and energy metabolism. These results encourage the translation of these biomarkers into in-vivo gastric cancer detection methodology using MRI-MS.
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