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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Citrinovirin with a new norditerpene skeleton from the marine algicolous fungus Trichoderma citrinoviride
Xiao-Rui Liang1, Feng-Ping Miao2, Yin-Ping Song3
1Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Naval Aeronautical and Astronautical University, Yantai 264001, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Citrinovirin (1), a novel norditerpene with an unprecedented carbon skeleton along with three known compounds, cyclonerodiol (2), 3-(2-hydroxypropyl)-4-(hexa-2E,4E-dien-6-yl)furan-2(5H)-one (3), and 5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone (4), was isolated from the culture of a marine brown alga-endophytic strain (cf-27) of Trichoderma citrinoviride. The structure and relative configuration of 1 were identified by spectroscopic methods, including 1D/2D NMR and MS. Its absolute configuration was established by analysis of ECD spectrum, aided by quantum chemical calculations. A plausible biogenetic pathway is proposed for 1, and it was evaluated to be active against Staphylococcus aureus.
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