Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diffuse Large B-cell Lymphoma Initially Managed As Secondary Immune Thrombocytopenia: A Case Report.

Cureus·2026
Same author

Wiskott-Aldrich Syndrome Across Three Siblings: Variable Clinical Expression and Current Understanding.

Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion·2026
Same author

Secondary Factor X Deficiency Associated with Sjögren's Syndrome: A Rare Case Report.

Current rheumatology reviews·2026
Same author

Fibroin/sulfated alginate membranes containing exosomes of stem cells treated with B and Zn-doped hydroxyapatite for periosteal tissue engineering.

Journal of materials chemistry. B·2026
Same author

Original Versus Generic Eltrombopag in Patients with Immune Thrombocytopenia: A Prospective Multi-Center Experience on Efficacy and Safety.

Journal of clinical medicine·2026
Same author

Injectable chitosan/maltodextrin/microcrystalline cellulose hydrogels loaded with melatonin for intervertebral disc regeneration.

International journal of biological macromolecules·2025

Related Experiment Video

Updated: Mar 15, 2026

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.4K

Crosslinked pullulan/cellulose acetate fibrous scaffolds for bone tissue engineering.

Deniz Atila1, Dilek Keskin2, Ayşen Tezcaner2

  • 1Department of Engineering Sciences, Middle East Technical University, Turkey.

Materials Science & Engineering. C, Materials for Biological Applications
|September 11, 2016
PubMed
Summary

New pullulan and cellulose acetate scaffolds show promise for bone tissue engineering. Crosslinking improved mechanical properties and stability, supporting cell growth and differentiation for potential bone graft applications.

Keywords:
Bone tissue engineeringCellulose acetatePullulanWet electrospinning

More Related Videos

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

2.9K
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.2K

Related Experiment Videos

Last Updated: Mar 15, 2026

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.4K
Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

2.9K
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.2K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Natural polymer scaffolds are explored for bone tissue engineering but often lack 3D structure and mechanical strength.
  • Pullulan and cellulose acetate are polysaccharides with potential for scaffold development.

Purpose of the Study:

  • To develop and characterize 3D fibrous scaffolds from pullulan and cellulose acetate blends for bone tissue engineering.
  • To enhance scaffold properties through crosslinking with trisodium trimetaphosphate (STMP).

Main Methods:

  • Electrospinning of various pullulan/cellulose acetate ratios (P/CA).
  • Crosslinking with STMP to improve mechanical properties and reduce weight loss.
  • Characterization of fiber morphology, mechanical properties, and stability in phosphate-buffered saline (PBS).
  • Bioactivity assessment using simulated body fluid (SBF) incubation and cell culture with Saos-2 cells.

Main Results:

  • P50/CA50 scaffolds exhibited uniform fiber morphology and efficient crosslinking.
  • Crosslinked P50/CA50 scaffolds showed improved mechanical properties, stability, and pore uniformity.
  • SBF incubation led to apatite-like structure formation.
  • Saos-2 cells adhered, proliferated, and exhibited osteoblastic differentiation on the scaffolds, indicating cytocompatibility.

Conclusions:

  • Crosslinked pullulan/cellulose acetate (P50/CA50) scaffolds are mechanically robust and cytocompatible.
  • These scaffolds demonstrate potential as cell carriers for bone tissue engineering applications.
  • The developed scaffolds offer a promising alternative for bone regeneration strategies.