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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Using AlphaScreen(®) to Identify Small-Molecule Inhibitors Targeting a Conserved Host-Pathogen Interaction.

Sisley Austin1, Saïd Taouji2, Eric Chevet3,4

  • 1Intracellular Transport of Viral Structures, MFP CNRS UMR 5234, University of Bordeaux, 146 rue Leo Saignat, Bat. 3A, 3eme etage, Bordeaux, 33076, France.

Methods in Molecular Biology (Clifton, N.J.)
|September 11, 2016
PubMed
Summary

AlphaScreen(®) technology enabled screening for inhibitors of a viral protein interaction. Researchers identified small molecules that specifically inhibit the interaction between Nedd4.2 and the adenoviral protein VI PPxY motif.

Keywords:
384-Well plateAdenovirusAlphaScreen®AntiviralHigh-throughputProtein-Protein interactionsSmall-molecule inhibitorsUbiquitin ligase Nedd4[LP]PxY motif

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Area of Science:

  • Biochemistry
  • Drug Discovery
  • Virology

Background:

  • AlphaScreen(®) is a bead-based proximity assay ideal for screening bi-molecular interactions.
  • The WW domains of Nedd4 family ubiquitin ligases interact with the PPxY motif found in viral proteins.
  • This interaction represents a potential drug target for antiviral therapies.

Purpose of the Study:

  • To develop and utilize an AlphaScreen assay to identify inhibitors of the Nedd4.2 WW domain and adenoviral protein VI PPxY motif interaction.
  • To screen a small molecule library against this specific protein-protein interaction.

Main Methods:

  • An AlphaScreen(®) assay was established to detect the interaction between Nedd4.2 and the PPxY motif of adenoviral capsid protein VI.
  • A library of small molecules was screened using this assay.

Main Results:

  • The AlphaScreen(®) assay successfully recapitulated the Nedd4.2-PPxY interaction.
  • Specific small molecule inhibitors of this interaction were identified through the screening process.

Conclusions:

  • The Nedd4.2-PPxY interaction is a viable target for drug discovery.
  • AlphaScreen(®) is an effective platform for identifying inhibitors of viral protein-protein interactions.