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[Biosynthesis of 14C-macrocyclic trichothecenes]
Mikrobiologicheskii Zhurnal
|March 1, 1989
Summary
Researchers successfully produced carbon-14 labeled macrocyclic trichothecenes (MCTC) with high specific activity using Dendrodochium toxicum. Labeled acetate introduced at the end of fungal growth yielded the best results for MCTC production.
Area of Science:
- Mycotoxicology
- Natural Product Synthesis
- Isotope Labeling
Context:
- Macrocyclic trichothecenes (MCTC) are toxic secondary metabolites with complex structures.
- Producing radiolabeled MCTC is crucial for metabolic and toxicological studies.
- Dendrodochium toxicum is a known producer of MCTC.
Purpose:
- To investigate the feasibility of producing (14C)-labeled macrocyclic trichothecenes (MCTC) with high specific activity.
- To optimize the precursor feeding strategy for enhanced radiolabeling of MCTC.
- To determine the specific activity of obtained (14C)-MCTC.
Summary:
- The study explored the production of (14C)-MCTC using (1-14C)-glucose, (2-14C)-acetate, and (2-14C)-mevalonate as precursors.
- Optimal results were achieved by introducing labeled acetate at the end of the logarithmic growth phase of Dendrodochium toxicum 5800.
- High specific activities were obtained for individual (14C)-MCTC, including verrucarine A (158.1 microC/mM), rhoridine A (167.4 microC/mM), and rhoridine H (161.9 microC/mM).
Impact:
- This research provides a method for generating high-specific-activity radiolabeled MCTC.
- The availability of these labeled compounds facilitates further research into MCTC biosynthesis, metabolism, and toxicity.
- Enables advanced analytical techniques and tracer studies involving MCTC.