Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Detection of anti-drug antibodies during monoclonal antibody drug therapy.

Trends in biotechnology·2026

Same author

Cross-continental transmission and host adaptation of <i>Mycobacterium tuberculosis</i> in China unveiled by population history reconstruction and adaptive evolution signal detection.

iMetaOmics·2026

Same author

Estimating Toxicity Putative Mechanisms from Smoking Residual Substances Using a Whole-Cell Bioreporter System.

Biosensors·2025

Same author

Whole-Cell Bioreporter-Based Assay for Detecting Fungal-Derived β-Lactamase Inhibitors.

Biosensors·2025

Same author

Drug-Tolerant, Chemiluminescent Lateral Flow Immunoassay Platform for the Determination of Neutralizing Anti-Drug Antibodies.

Small (Weinheim an der Bergstrasse, Germany)·2025

Same author

Whole-Cell Fiber-Optic Biosensor for Real-Time, On-Site Sediment and Water Toxicity Assessment: Applications at Contaminated Sites Across Israel.

Biosensors·2025

Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026

Same journal

A novel capillary-driven dual-mode imaging flow cytometry system for malaria parasite detection and quantification.

Biosensors & bioelectronics·2026

Same journal

Liver-targeted alkaline phosphatase-activatable fluorescent probe for imaging liver fibrosis and screening anti-fibrotic natural products.

Biosensors & bioelectronics·2026

Same journal

GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics.

Biosensors & bioelectronics·2026

Same journal

CRISPR/Cas12a-based dual-modal signal platform using MIL-101(Fe) for colorimetric and electron spin resonance detection of HPV-16 nucleic acid.

Biosensors & bioelectronics·2026

Same journal

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

Biosensors & bioelectronics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 15, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Colorimetric stack pad immunoassay for bacterial identification.

Evgeni Eltzov¹, Robert S Marks²

¹School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore; Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; NTU-HUJ-BGU CREATE Programme, 1 Create Way, Research Wing #02-06 to 08, 138602, Singapore.

Biosensors & Bioelectronics

|September 13, 2016

Summary

This summary is machine-generated.

A novel stacked lateral flow immunoassay offers a 1,000-fold increase in sensitivity for detecting bacteria like E. coli. This rapid, simple assay provides accurate results in under 5 minutes.

Keywords:

Bacteria Colorimetry Immunoassay Membranes Miniaturization

More Related Videos

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Bacterial Detection & Identification Using Electrochemical Sensors

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Related Experiment Videos

Last Updated: Mar 15, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Bacterial Detection & Identification Using Electrochemical Sensors

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Area of Science:

Biotechnology
Analytical Chemistry
Biosensing

Background:

Conventional lateral flow assays (LFAs) are widely used for rapid diagnostics but often lack sufficient sensitivity.
Improving the sensitivity of LFAs is crucial for early detection of low-concentration analytes, such as bacteria in food or water samples.

Purpose of the Study:

To develop a novel colorimetric immunoassay concept with enhanced sensitivity for bacterial detection.
To investigate a stacked lateral flow membrane configuration for improved analyte capture and signal amplification.

Main Methods:

A stacked lateral flow assay was designed using conventional membranes in a vertical configuration, with sample flow from bottom to top.
A capture layer of immobilized target bacteria (Escherichia coli) was incorporated for specific analyte binding.
Horseradish peroxidase (HRP)-conjugated antibodies were used for signal generation via enzymatic reaction.

Main Results:

The stacked assay demonstrated a sensitivity of 10^2 cells/mL for E. coli detection, which is 1,000-fold higher than traditional ELISAs.
The assay achieved a positive colorimetric signal within 5 minutes.
The assay design effectively prevented non-specific binding and ensured efficient capture of target bacteria.

Conclusions:

The novel stacked lateral flow immunoassay offers a significant advancement in sensitivity and speed for bacterial detection.
This technology presents advantages of miniaturization, operational simplicity, and rapid response time.
The enhanced sensitivity makes it suitable for detecting low bacterial loads in various sample types.