Redefining Microascus, Scopulariopsis and allied genera

Area of Science:

• Mycology and Fungal Taxonomy
• Molecular Phylogenetics
• Environmental Microbiology

Background:

• The genera Microascus and Scopulariopsis include species found in soil, decaying matter, and indoor environments.
• Some species are opportunistic pathogens affecting insects, animals, and humans.
• Previous taxonomy relied solely on morphology, leading to potential inaccuracies.

Purpose of the Study:

• To clarify the taxonomy and phylogeny of Microascus and Scopulariopsis species.
• To delineate species using integrated morphological, physiological, and molecular analyses.
• To provide a robust classification framework for these commonly encountered fungi.

Main Methods:

• Studied a large collection of clinical and environmental isolates, including ex-type strains.
• Employed morphological, physiological, and molecular analyses.
• Utilized Genealogical Phylogenetic Species Recognition (GCPSR) with DNA sequence data from four loci (ITS, rDNA LSU, translation elongation factor 1-α, β-tubulin).

Main Results:

• Established that Microascus and Scopulariopsis represent two distinct phylogenetic lineages.
• Reinstated the genus Pithoascus and erected the new genus Pseudoscopulariopsis.
• Described seven new species of Microascus and one new Scopulariopsis species, with further taxonomic revisions including new combinations and neotypifications.

Conclusions:

• The study provides a revised and phylogenetically supported classification for Microascus and Scopulariopsis.
• New genera and species are introduced, enhancing the accuracy of fungal identification.
• The findings contribute to a better understanding of the diversity and evolutionary relationships within these fungal groups.