Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Modeling Approach to Inform In Vitro Hypoxia Platforms for Patient-Specific Analyses.

Annals of biomedical engineering·2026
Same author

In vitro sex-specific function-structure relationship in neonatal rat cardiac monolayers.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Adaptive response to electrical pulse stimulation is impaired in FSHD myotubes by DUX4 gene network activation.

Scientific reports·2025
Same author

Biaxial length-tension relationship in single cardiac myocytes.

Biophysical journal·2025
Same author

Improving the cardiovascular outcomes of obstructive sleep apnea: Towards more precise hypoxia-based models of disease severity.

Current sleep medicine reports·2025
Same author

Polarized macrophages modulate cardiac structure and contractility under hypoxia in novel immuno-heart on a chip.

APL bioengineering·2025

Related Experiment Video

Updated: Mar 15, 2026

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

7.6K

Multiscale Characterization of Engineered Cardiac Tissue Architecture.

Nancy K Drew1, Nicholas E Johnsen2, Jason Q Core2

  • 1Department of Biomedical Engineering, Center for Complex Biological Systems, The Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, Irvine, CA 92697

Journal of Biomechanical Engineering
|September 13, 2016
PubMed
Summary

This study quantifies cardiac tissue architecture using novel metrics, revealing how cellular shape influences self-assembly and identifying key organization scales in cardiovascular tissues.

More Related Videos

Capillary Force Lithography for Cardiac Tissue Engineering
10:09

Capillary Force Lithography for Cardiac Tissue Engineering

Published on: June 10, 2014

13.0K
Author Spotlight: Advancing Human Cardiac Anatomy Through Multi-Scale Analysis of Hearts
04:22

Author Spotlight: Advancing Human Cardiac Anatomy Through Multi-Scale Analysis of Hearts

Published on: June 28, 2024

1.0K

Related Experiment Videos

Last Updated: Mar 15, 2026

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

7.6K
Capillary Force Lithography for Cardiac Tissue Engineering
10:09

Capillary Force Lithography for Cardiac Tissue Engineering

Published on: June 10, 2014

13.0K
Author Spotlight: Advancing Human Cardiac Anatomy Through Multi-Scale Analysis of Hearts
04:22

Author Spotlight: Advancing Human Cardiac Anatomy Through Multi-Scale Analysis of Hearts

Published on: June 28, 2024

1.0K

Area of Science:

  • Biophysics
  • Tissue Engineering
  • Cardiovascular Mechanics

Background:

  • Cardiac muscle exhibits intricate subcellular architecture crucial for contraction.
  • Pathological conditions alter cardiac architecture, but scale-dependent relationships remain underexplored.
  • Quantitative metrics are needed to understand cardiac tissue organization.

Purpose of the Study:

  • To develop and apply novel architecture metrics for quantifying organization and consistency in cardiac cells and tissues.
  • To explore the relationship between cellular shape, self-assembly, and structural organization across multiple length-scales.
  • To investigate the correlation between the organization of actin, tubulin, sarcomeric z-lines, and nuclei in cardiac constructs.

Main Methods:

  • Utilized orientational and co-orientational order parameters (COOPs) to quantify structural organization.
  • Applied metrics to engineered neonatal rat ventricular myocytes and cardiac tissues (isotropic and anisotropic).
  • Stained cells and tissues for actin, tubulin, sarcomeric z-lines, and nuclei for detailed analysis.

Main Results:

  • Established a link between cellular shape and the consistency of self-assembly in cardiac constructs.
  • Determined the characteristic length-scales at which unguided cardiac tissues self-organize.
  • Quantified the correlation (or lack thereof) between the organization of actin, tubulin, sarcomeric z-lines, and nuclei.

Conclusions:

  • Novel quantitative metrics provide new insights into cardiac tissue architecture and self-assembly.
  • Findings elucidate mysteries in the relationship between force production and cardiac structure.
  • These metrics represent a future direction for quantitative tissue engineering in cardiovascular biomechanics.