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Related Concept Videos

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Regulation of Expression Occurs at Multiple Steps02:24

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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Chromatin Structure Regulates pre-mRNA Processing02:41

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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RNA Polymerase II Accessory Proteins02:36

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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Related Experiment Video

Updated: Mar 15, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
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Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

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AUF1 regulation of coding and noncoding RNA.

Elizabeth J F White1,2, Aerielle E Matsangos1,2, Gerald M Wilson1,2

  • 1Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA.

Wiley Interdisciplinary Reviews. RNA
|September 14, 2016
PubMed
Summary
This summary is machine-generated.

The RNA-binding protein AUF1 (AU-rich element binding protein 1) has diverse roles beyond mRNA stability, including regulating microRNA synthesis and interacting with noncoding RNAs.

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Area of Science:

  • Molecular Biology
  • RNA Biology
  • Gene Expression Regulation

Background:

  • AUF1 (AU-rich element binding protein 1) is an RNA-binding protein family involved in mRNA stability and translation.
  • Canonical functions of AUF1 are linked to AU-rich elements in 3' untranslated regions of mRNAs.
  • Recent transcriptomic studies reveal broader roles for AUF1 in posttranscriptional gene regulation.

Purpose of the Study:

  • To review recent advancements in understanding AUF1's RNA-binding properties and biochemical implications.
  • To explore AUF1's newly discovered roles in microRNA (miRNA) synthesis and function.
  • To discuss AUF1's interactions with long noncoding RNAs and viral RNAs.

Main Methods:

  • Literature review of recent studies on AUF1.
  • Analysis of AUF1's biochemical properties and target interactions.
  • Survey of AUF1's involvement in miRNA biogenesis and function.

Main Results:

  • AUF1 exhibits diverse RNA-binding capabilities influencing mRNA decay and translation.
  • AUF1 plays roles in miRNA synthesis, miRISC assembly, and targeting.
  • AUF1 interacts with other RNA-binding proteins, noncoding RNAs, and viral RNAs.

Conclusions:

  • AUF1's functions extend significantly beyond its canonical roles in mRNA regulation.
  • AUF1 is a key regulator in a complex network involving miRNAs, lncRNAs, and viral RNAs.
  • Emerging research on AUF1 opens new avenues for understanding posttranscriptional gene control.