Glypican4 modulates lateral line collective cell migration non cell-autonomously
Marina Venero Galanternik1, Mark E Lush2, Tatjana Piotrowski1
1Stowers Institute for Medical Research, Kansas City, MO 64110, USA; Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84132, USA.
Developmental Biology
|September 20, 2016
Summary
Glypican4 is crucial for zebrafish lateral line development, guiding collective cell migration. Its loss disrupts Wnt/Fgf signaling and muscle precursor development, essential for proper cell movement.
Area of Science:
- Developmental Biology
- Cell Biology
- Genetics
Background:
- Collective cell migration is vital for embryonic development and cancer metastasis.
- Proteoglycans regulate signaling pathways controlling cell migration.
- The zebrafish lateral line system is a model for studying collective cell migration.
Purpose of the Study:
- To investigate the function of glypican4 in zebrafish lateral line development.
- To understand how glypican4 coordinates cell-intrinsic and environmental cues during migration.
Main Methods:
- Analysis of glypican4 expression patterns in zebrafish.
- Phenotypic characterization of glypican4 (knypekfr6) mutants.
- Investigation of signaling pathways (Wnt/β-catenin, Fgf, Hh) and cell interactions.
Main Results:
- Glypican4 mutants exhibit severe defects in lateral line primordium migration.
- Glypican4 regulates Wnt/β-catenin/Fgf signaling feedback redundantly with other proteoglycans.
- Primordium migration defects are non-cell autonomously caused by loss of cxcl12a+ muscle precursors via Hh downregulation.
Conclusions:
- Glypican4 plays essential, distinct roles in both primordium cells and their environment.
- Both cell-autonomous and non-cell-autonomous functions of glypican4 are critical for collective cell migration.
- This study elucidates the complex regulation of collective cell migration by proteoglycans in a vertebrate model.
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